By Maria G Gallegos
Much is known of human pathogenic bacteria however
less about Chlamydia intracellular
parasites species Rhabdochlamydia
porcellionis. Even though, it has
not been reported to cause human death emerging evidence suggest it being a
cause for bovine abortion and respiratory disease. It is known to cause serious
liver tissue damage due to its two stage developmental cycle compromising
formation of an intracellular reticulate body (RBs) and non-diving infectious
elementary body (EBs). Much remains unknown of this and other Chlamydiae species due to a lack of cell
culture based methodology needed to isolate and grow them. The goal of this research was to develop a
viable cell line where to grow and study the reproductive cycle. Findings cocultivation with amoeba
unsuccessful but viable with insect cell lines Sf9 from moth ovarian tissue. Much
remains to be discovered and now can be further investigated with the successful
maintenance of this parasite.
Figure 1 from Sixt et.al showing Sf9 cells infected
with R. porcellionis. Bacterial
replication monitoring of intracellular parasite with the use of confocal fluorescence
for 31 days shows an increase in replication as time progresses.
Original
article: Sixt B, Kostanjšek R, Mustedanagic A, Toenshoff E, Horn M. (2013). Developmental
cycle and host interaction of Rhabdochlamydia porcellionis, an intracellular parasite
of terrestrial isopods. Environmental Microbiology 15: 2980-2993.
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