By: Alejandra Pena
A. Likelihood phylogeny of Loki- and hemidallarchaeota operational taxonomic units and their related sequences from SILVA database. Specificities of FISH probes and the amount of sequence in each OTU are shown. B. Relative abundances of Loki- and Hemidallarchaeota sequences shown at different sediment depths. Figure taken from Avci et al. 2021
Asgard archaea are known prokaryotes, yet are known to be the most similar form of prokaryotes that show eukaryotic properties. This particular type of archaea shows similar genomes to signature eukaryotic proteins found in the first eukaryotic cell. Asgard archaea are very diverse, making the visualization of these cells in the environment crucial in the understanding of their structure and diversity. A primer free sequence was used in order to retrieve rRNA sequences from Loki- and Heimdallarchaeota to later design oligonucleotide probes and properly visualize cells in the marine sediments. The results show that the genomic material of both Loki- and Hemidallarchaeota detected in the cells are condensed and distinct in the riboplasm. This proved that the role of asgard archaea can be indicative of cellular compartmentalization. This method could be used in future research in order to use a reliable method of in situ imagine of rare microorganisms in environmental samples, as well as using the ultrastructure of asgard archaeal cells by using electron microscopy for insight on the complex structure of eukaryotic cells.
Reference:
Avcı, B., Brandt, J., Nachmias, D. et al. Spatial separation of ribosomes and DNA in Asgard archaeal cells. ISME J (2021). https://doi.org/10.1038/s41396-021-01098-3
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