Tuesday, September 3, 2013

Pseudomonas: We Control Thee

by: Patrick J. Palacios



Fossil fuel combustion, industrial pollution and other anthropogenic emissions arise every day.  These processes release several unwanted chemicals into our environment.  One of the most common and lethal: arsenic.  There are few known ways to sustainably remove the toxic substance; however, bacteria have proven to be of some assistance.  The arsM gene (responsible for arsenic methylation) from Chlamydomonas reinhardtii was inserted into Psuedomonas putida via conjugation.  P. putida was then subjected to 25uM arsenic and was allowed to “work its magic.”  It was found that P. putida successfully biotransformed arsenic to less toxic products.  This shows much potential in bioremediation of air and contaminated water sources which is a devastation that many countries suffer from.
Figure from Chen et al. 2013 showing levels of arsenic (As(III)) and products after P. putida exposure (DMA, MA and As(V)) over time.

 Original article: Chen J, Qin J, Zhu YG, deLorenzo V, Rosen BP. (2013). Engineering the soil bacterium psuedomonas putida for arsenic methylation. Appl. Environ. Microbiol. 79:4493-4495.




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