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| Figure 1: Growth using H2O and heavy water (B) is recorded. Each spectrum represented carbon source and growth conditions in single carbon sources, aromatics (ar), casamino acids (ca), peptone (pe), SCFA (fa), sugars (su) as well as a complex medium. Each box plot represents medium and upper and lower quartile (C) . The mean value of the relative deuterium amount of all six carbon sources conditions are represented with a black line. |
A rapid and reliable method for differentiation between active and inactive bacteria cells is urgently needed, one of these methods to identify active microbes is stable isotope probing or (SIP), where microbial communities are supplemented with substrates label with heavy, nonradioactive isotopes, resulting in the assimilation of these isotopes by active microorganisms. However, when combined with Raman micospectroscopy, a nondestructive technique for obtaining information about the molecular composition's of microorganisms at the single cell level, can be a potential way for a more effective approach to collect data about these microorganisms (Georgette et al. 2020) did a study based on the quantification of deuterium assimilation from heavy water (D2O) into single bacterial cells to check the influence of carbon source and bacterial identity on the deuterium uptake. During the study they showed that the presence of simple sugars had a higher presence but differ significantly among bacterial isolates. Despite this variability, the classification models could easily differentiate deuterium labeled and unlabeled single cells with high sensitivity and specificity. This shows that the Raman-D2O approach when combined with chemometrics, proves to be a great approach to monitoring single bacterial cells.
Original Article
Anal. Chem.2020, 92, 16, 11429–11437 Publication Date: July 22, 2020 https://doi.org/10.1021/acs.analchem.0c02443
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