Shotgun sequencing

One virus that happens to be a major threat to the public health and that is global is human pathogens. Many techniques have been developed to detect human pathogens in waste water treatment plants and to see the risks that they may can cause to the environment. This study was to use sets that would be derived with high sequencing technique for a shotgun to see if any activated sludge, to detect human pathogens, etc. Each set of data was filtered and the DNA sequence was normalized to a certain number and had a length of 150-190 bp. Different databases were used and one of the ones that was used was the 16S rRNA gene; the results showed that nine bacterial pathogens had been detected, with an overall amount that was 0.06-3.20% in the 16S rRNA gene fingerprinting, and sludge was activated as well. Pathogen risks were present because the sludge and effluent were detected and both were high in abundance. There is a concern in human pathogens and it was demonstrated that the sequencing technique for the shotgun was high and was feasible for a detection to the environment of human bacterial pathogens. There are many ways to detect human pathogens in waste water treatments and shotgun sequencing is one of the ways that’s been known to detect them.

 

Figure 1: Shotgun sequencing in a WWTP.

References:

Cai, L. and Zhang, T. 2013. “Detecting Human Bacterial Pathogens in Wastewater Treatment Plants by a High-Throughput Shotgun Sequencing Technique.” Environmental Science & Technology 47 (10), pp 5433–5441